Histologically, chalazion shows a typical appearance of lipogranulomatous
reaction - a form of chronic inflammatory reaction. Once you have made
the diagnosis, the examiner usually moves to a discussion of cells that
are seen in chronic inflammation especially the types of giant cells which
may give clue to the underlying condition.
The features to look for are:
multiple inflammatory cells surrounding clear spaces
(the clear space is the result of lipid which has been removed during processing).
presence of multinucleated giant cells.
Low magnification H&E
Presence of inflammatory cells surrounding a clear space
(where lipid has been removed during tissue preparation).
The inflammation is a foreign body response to lipid
leaks out of sebaceous glands or the glands of Zeiss.
High magnification H & E
Giant cells (arrowed) in chalazion.
Common questions in the viva: