Test 13 (biochemistry)
1. The following are true about DNA synthesis:
a. it requires DNA polymerase
b. reverse transcriptase enzymes are involved
c. moves in a 5'---> 3' direction
d. the rate of error in DNA synthesis is 1 in 105 base pairs
e. cytarabine inhibits DNA synthesis

2. With regard to DNA molecules:
a. they contain adenine, cytosine, guanine and uracil bases
b. they can be detected with Western blotting
c. they can be detected with Southern blotting
d. they are denatured at temperature of 1000C
e. they are denatured by pH of  £ 7.14


3. G-proteins:
a. are activated by the binding of an extracellular ligand to a membrane receptor
b. can be mutated in tumour cells
c. mediate the action of glucocorticoid hormone
d. they are inactivated by cholera toxins.
e. bind to DNA to regulate gene transcription


4. The following is true about gluconeogenesis:
a. it occurs in liver
b. it occurs in kidney
c. it occurs in adipose tissue
d. it is inhibited by glucagon
e. it is stimulated by insulin

5. With regard to membrane receptors for hormones:

a. they are often glycoproteins
b. they are important for hormones made up of steroid
c. those for insulin exhibit an intrinsic protein kinase activity
d. glucagon uses calcium as a second messenger
e. catecholamines uses cyclic AMP as second messenger


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